Plasmid

Part:BBa_K5120014:Design

Designed by: Ananya Anuj Dharna   Group: iGEM24_Thailand-RIS   (2024-10-01)


Isoflavanoid Synthase (IFS) in pEAQ DEST 1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 10226
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 5173
    Illegal NotI site found at 10232
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 10226
    Illegal BglII site found at 644
    Illegal BglII site found at 1657
    Illegal BglII site found at 1913
    Illegal BglII site found at 1933
    Illegal BglII site found at 8791
    Illegal BglII site found at 9775
    Illegal BamHI site found at 1146
    Illegal XhoI site found at 714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 10226
    Illegal suffix found in sequence at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 10226
    Illegal XbaI site found at 10241
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 2397
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3636
    Illegal NgoMIV site found at 4168
    Illegal NgoMIV site found at 5045
    Illegal NgoMIV site found at 5169
    Illegal NgoMIV site found at 6196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-Some silent mutations were made to meet the part assembly standards, -pEAQ-DEST1 was modified to include a suffix and prefix



Source

The code for pEAQ-DEST1 was taken from ncbi database, The gene for IFS comes from Puearira Mirific Var. Lobata